LysR-type transcriptional regulator <i>CARR</i> represses the expression of <i>bla</i> _CAR-2 and reduces <i>P. diazotrophicus</i> resistance to cefalothin, cefuroxime and cefotaxime.

ABSTRACT:

Background

P. diazotrophicus was isolated from a newborn with D-galactosemia complicated sepsis. A homologous bla _CAR-2 gene, encoding CAR-2, a predicted member of the CAR family subclass B3 metallo-β-lactamases (MBLs), was found in the genome of this strain. This study aimed to explore the identification of a novel CAR-2 protein encoded by the chromosome of P. diazotrophicus Pd1 that exhibits the zinc-binding motifs of subclass B3 enzymes and the regulatory pattern of CARR, located directly upstream of the bla _CAR-2 gene, on the bla _CAR-2 gene and its impact on antibiotic resistance.

Methods

Antibiotic susceptibility testing was conducted by the plate agar dilution method. Site-directed mutagenesis w

SUBMITTER: Lin J 

PROVIDER: S-EPMC12434095 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature