Differential Capacitance Spectroscopy for Real-Time Monitoring of RNA Amplification.

ABSTRACT: RNA amplification is central to viral diagnostics, yet current optical and fluorometric methods, such as PCR and RT-qPCR, remain costly, complex, and resource-intensive. Here, we introduce differential capacitance spectroscopy (DCS) as a real-time electrochemical method for RNA detection using loop-mediated isothermal amplification. By applying sinusoidal currents to flexible carbon fiber electrodes (0.05 cm², 1 mm apart), DCS monitors changes in electrode-electrolyte interface capacitance, generating a unique arm-shoulder diagram (ASD) for RNA amplification. The ASD reveals exponential and polynomial capacitance variations, distinguishing amplified RNA under isothermal conditions. Our method eliminates the need for sophisticated instrumentation and reduces diagnostic costs whil

SUBMITTER: Nascimento SQ 

PROVIDER: S-EPMC12434665 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature