Coaxial Electrospraying for Black Seed Oil Nanoencapsulation: Improved Thymoquinone Stability and Bioactivity.

ABSTRACT: Thymoquinone is the primary bioactive compound in black seed oil (BSO), but it has limited stability under harsh conditions. Nanoencapsulation using zein via coaxial electrospraying is an effective approach to improve its stability and bioactivity during gastrointestinal transit. Therefore, this study aimed to develop BSO-loaded nanoparticles using coaxial electrospraying, evaluate their stability at different temperatures (4°C, 25°C and 60°C), and investigate their in vitro bioaccessibility, intestinal permeability using Caco-2 cell models, and immunomodulatory potential. Nanoparticles with high encapsulation efficiency (74.38% ± 2.6%) and loading capacity (12.99% ± 3.6%) retained thymoquinone content at 91.3%, 74.7%, and 52.0% after 55 days of storage at 4°C, 25°C, and 60°C, respectively

SUBMITTER: Atay E 

PROVIDER: S-EPMC12434836 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature