Spatial TCR clonality and clonal expansion in the in situ microenvironment of non-small cell lung cancer.

ABSTRACT:

Background

T-cell activation and clonal expansion are essential to effective immunotherapy responses in non-small cell lung cancer (NSCLC). The distribution of T-cell clones may offer insights into immunogenic mechanisms and imply potential prognostic and predictive information.

Methods

We analyzed α/β T-cell receptor (TCR) clonality using RNA-sequencing of bulk frozen tumor tissue from 182 patients with NSCLC. The data was integrated with molecular and clinical characteristics, extensive in situ imaging, and spatial sequencing of the tumor immune microenvironment. TCR clonality was also determined in an independent cohort of nine patients with immune checkpoint-treated NSCLC.

Results

TCR clonality (Gini index) patterns ranged from high T-cell clone diversity with hig

SUBMITTER: Yu H 

PROVIDER: S-EPMC12434955 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature