Efficient Expression and Purification of Recombinant Mouse Dimeric IgA.

ABSTRACT: Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single-cell level.

SUBMITTER: Geisse A 

PROVIDER: S-EPMC12435132 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature