¹⁹F NMR-tags for peptidyl prolyl conformation analysis.

ABSTRACT: Proline cis/trans isomerism plays an important role in protein folding and mediating protein-protein interactions in short linear interacting motifs within intrinsically disordered protein regions. The slow exchange rate between cis and trans prolyl bonds provides distinct signals in ¹⁹F NMR analysis of fluorinated peptides, allowing for simple quantification of each population. However, fluorine is not naturally found in proteins but can be introduced using chemical tags. In this study, we evaluate a range of fluorinated cysteine-reactive ¹⁹F NMR tags to assess their ability to react with short, linear proline-containing peptides and accurately report on the equilibrium cis/trans-Pro populations. Several fluorinated electroph

SUBMITTER: Hanson GSM 

PROVIDER: S-EPMC12435502 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature