An Arabidopsis gene encoding an alpha-xylosyltransferase involved in xyloglucan biosynthesis.

ABSTRACT: Microsomal membranes catalyze the formation of xyloglucan from UDP-Glc and UDP-Xyl by cooperative action of alpha-xylosyltransferase and beta-glucan synthase activities. Here we report that etiolated pea microsomes contain an alpha-xylosyltransferase that catalyzes the transfer of xylose from UDP-[(14)C]xylose onto beta(1,4)-linked glucan chains. The solubilized enzyme had the capacity to transfer xylosyl residues onto cello-oligosaccharides having 5 or more glucose residues. Analysis of the data from these biochemical assays led to the identification of a group of Arabidopsis genes and the hypothesis that one or more members of this group may encode alpha-xylosyltransferases involved in xyloglucan biosynthesis. To evaluate this hypothesis, the candidate genes were expressed in Pichia past

SUBMITTER: Faik A 

PROVIDER: S-EPMC124356 | biostudies-literature | 2002 May

REPOSITORIES: biostudies-literature