Impairment in global protein synthesis uncouples UPR gene induction from <i>HAC1</i> mRNA splicing in <i>Saccharomyces cerevisiae</i>.

ABSTRACT: Upon dysfunction of the endoplasmic reticulum (ER), also known as ER stress, eukaryotic cells alter their transcriptomes. This cytoprotective response is called the unfolded protein response (UPR), which is mediated by Ire1 and HAC1 in the yeast Saccharomyces cerevisiae. ER stress induces self-association and activation of the ER-resident transmembrane endoribonuclease Ire1, which catalyzes the splicing of HAC1 mRNA. It is widely accepted that HAC1 mRNA is translated into the nuclear transcription factor Hac1, only after being spliced. To investigate the cellular response to ethanol-induced ER stress, here we gradually added ethanol into S. cerevisiae cultures until reaching a final concentration of 16%. Unlike conventional ER stressors, such as tunicamyc

SUBMITTER: Geronimo RAC 

PROVIDER: S-EPMC12435717 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature