Depletion of <i>Mycobacterium tuberculosis</i> transmembrane protein Rv3737 reduces pathogen survival and induces M1 macrophage polarization against tuberculosis.

ABSTRACT:

Objectives

Mycobacterium tuberculosis (Mtb) modulates macrophage polarization to evade host immunity and enhance intracellular survival. Rv3737, a probable conserved transmembrane protein in Mtb, has an unclear biological function. This study investigates the role of Rv3737 in regulating macrophage polarization and Mtb survival within host cells.

Methods

The structure of Rv3737 was predicted using bioinformatics tools. Macrophage polarization markers were assessed by real-time PCR for M1/M2-associated cytokines, and flow cytometry for CD86⁺/CD206⁺ expression. RNA sequencing, along with KEGG and GO analyses, was used to explore underlying regulatory pathways. Western blotting evaluated the phosphorylation status of NF-κB (P65, IκB) and MAPK (ERK,

SUBMITTER: Peng Z 

PROVIDER: S-EPMC12436412 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature