Ischemia-reperfusion injury with a model of porcine whole-blood <i>ex-vivo</i> lung perfusion.

ABSTRACT:

Introduction

Our objective was to model Ischemia-Reperfusion (IR) injuries by ex-vivo perfusion of porcine lungs with whole blood containing the inflammatory cells.

Methods

Lungs and whole blood were collected from 12 pigs and submitted to cold ischemia time (CIT) of 1 or 18 h. The lungs were then ventilated and perfused for 6 h at 37°C using donor whole blood. Pulmonary pressure was 20 mmHg.

Results

Compared to the short CIT group, the long CIT group had a lower maximum perfusion flow rate (mean difference in % cardiac output, -39%; 95% CI, -66 to -12; P = 0.005) and higher pulmonary vascular resistance (mean difference, 1,077 dyne·s·cm^-⁵; 95% CI, 685-1,469; P < 0.001). Neutrophils decreased more in the long CIT group (mean difference,

SUBMITTER: Menager JB 

PROVIDER: S-EPMC12436443 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature