Probing three-dimensional cyclooctatetraene for nucleobase modification in aptamer selection.

ABSTRACT: Decoration of aptamers with chemical modifications at the level of nucleobases grants access to alternative binding modes, which often result in improved binding properties. Most functional groups involved in such endeavours mimic the side chains of amino acids or are based on sp²-dominated moieties. While this approach has met undeniable success, trends in modern drug discovery seem to favor sp³-rich compounds over aromatic derivatives. Here, we report the use of a nucleotide modified with the three-dimensional, highly flexible cyclooctatetraene carboxylate (COTc). This nucleotide was engaged in an SELEX experiment against the biomarker PvLDH. Tightly binding aptamers were identified, which displayed dissociation constants in the low nM range, representing a signific

SUBMITTER: Dahm GC 

PROVIDER: S-EPMC12436594 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature