Genome-wide estimation of transcript concentrations from spotted cDNA microarray data.

ABSTRACT: A method providing absolute transcript concentrations from spotted microarray intensity data is presented. Number of transcripts per microg total RNA, mRNA or per cell, are obtained for each gene, enabling comparisons of transcript levels within and between tissues. The method is based on Bayesian statistical modelling incorporating available information about the experiment from target preparation to image analysis, leading to realistically large confidence intervals for estimated concentrations. The method was validated in experiments using transcripts at known concentrations, showing accuracy and reproducibility of estimated concentrations, which were also in excellent agreement with results from quantitative real-time PCR. We determined the concentration for 10,157 genes in cervix canc

SUBMITTER: Frigessi A 

PROVIDER: S-EPMC1243803 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature