Simulations reveal a balance between protein-protein and protein-lipid interactions during condensation on membrane surfaces.

ABSTRACT: Liquid-liquid phase separation of protein condensates occurs frequently on biological membranes, where it is involved in diverse physiological processes from cell-cell recognition to endocytosis. Several recent studies have suggested that binding to lipids promotes phase separation of proteins. However, relatively little is known about the underlying molecular mechanisms. Here we use coarse-grained molecular dynamics simulations, grounded by data from experiments, to investigate the condensation of intrinsically disordered proteins on membrane surfaces. Attaching polyampholytic intrinsically disordered proteins to membranes composed of lipids with neutral head groups resulted in spontaneous protein condensation and coarsening on membrane surfaces, in agreement with experimentally-derived p

SUBMITTER: Zinga K 

PROVIDER: S-EPMC12439209 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature