Experimental conditions shape <i>in vitro</i> formation of murine platelet-leukocyte aggregates.

ABSTRACT:

Background

Platelets interact with leukocytes to fine-tune their functions, thus providing essential regulation of (patho-) physiologic immune responses in various diseases. Circulating platelet-leukocyte aggregates (PLAs) represent a sensitive biomarker to estimate disease severity both in patients and murine models. However, a limited understanding of the sensitivity of PLA measurements to methodological variables may undermine their accuracy and comparability.

Objectives

To elucidate how blood draw techniques, anticoagulation, processing delay and assay temperature affect murine platelet-leukocyte interactions.

Methods

Murine blood was obtained via retro-orbital, vena cava or cardiac puncture, anticoagulated with heparin, citrate or acid-citrate-dextrose (AC

SUBMITTER: Trivigno SMG 

PROVIDER: S-EPMC12439260 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature