The first abdominal aortic aneurysm organoid model replicates complex microenvironment for <i>in vitro</i> disease study.

ABSTRACT: Background: Abdominal Aortic Aneurysm (AAA) is a critical global health issue, affecting an estimated up to 8% of men over 65, with a complex etiology involving smoking, age and gender. However, the lack of specific drug treatments underscores the need for a robust in vitro model to advance our comprehension of AAA pathophysiology and serve as an ex vivo surrogate for drug testing. Methods: This study introduces an innovative AAA patient-derived organoid (PDO) model using a non-enzymatic procedure, a Matrigel-free system, and specialized organoid culture medium, leveraging 3-dimensional (3D) cultures to replicate the disease's microenvironment. The stability of this culture system was assessed through microscopic observation, H&E staining, immunohistochemistry (IHC), viabilit

SUBMITTER: Feng J 

PROVIDER: S-EPMC12439346 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature