Optimization of <i>q</i> Value for Sensitive Detection of Uridine and Thymidine Nucleosides by MS³.

ABSTRACT: Liquid chromatography-tandem mass spectrometry has been widely used to quantify modified nucleosides enzymatically released from RNA and DNA. Compared to MS/MS, MS³ offers higher specificity for analytes in complex sample matrices because coeluting chemical interferences can be more effectively filtered out. To date, the detection of uracil- and thymine-containing nucleosides and their modified derivatives still suffers from poor sensitivity, owing, in part, to their relatively low proton affinities. In this study, we explored various settings of q values in the MS³ analysis of unmodified and chemically modified uridine and thymidine nucleosides on a linear ion trap mass spectrometer. Our results showed that increasing the q value led to markedly improve

SUBMITTER: Duan Z 

PROVIDER: S-EPMC12439433 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature