Neutrophil extracellular traps induce endothelial damage and exacerbate vasospasm in traumatic brain injury.

ABSTRACT: Cerebral vasospasm (CVS) critically exacerbates secondary brain injury following traumatic brain injury (TBI). Understanding the underlying mechanisms is essential for developing targeted interventions. Methods: We developed a comprehensive murine multimodal imaging platform to evaluate CVS cerebral perfusion, and blood-brain barrier (BBB) integrity, integrating in vivo multiphoton microscopy, magnetic resonance angiography, carotid Doppler ultrasound, and laser speckle contrast imaging with molecular assays and functional assessments. Additionally, we comprehensively analyze single-cell RNA (TBI vs Sham) and bulk-RNA data (NETs-treated vs Control), delineating NETs-driven endothelial injury signatures. Finally, we explored the roles of PAD4^-/-, TLR4 inhibition and

SUBMITTER: Wang J 

PROVIDER: S-EPMC12439479 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature