Transgenic barley over-expressing <i>Aspergillus niger</i> phytase phyA in field trials.

ABSTRACT: Phytic acid is the main storage of phosphate in grains of staple crops. As phytic acid is hardly digestible for non-ruminants microbial phytases are used to supplement animal feed to enhance phosphate digestibility. A fungal phytase gene was introduced into barley with the aim of enhancing phosphate digestibility. Transgenic homozygous barley over-expressing fungal phytase phyA showed a 3.3fold increase in mature grain phytase activity. Field trials at two locations in the Czech Republic were conducted in a five-year experiment to test transgene stability and activity under field conditions. Increased phytase activity gradually decreased over the generations showing the most significant drop in the initial years of field trials. Molecular analysis revealed methylation in the coding sequenc

SUBMITTER: Vlcko T 

PROVIDER: S-EPMC12439572 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature