Single-cell transcriptomic profiling of <i>C. elegans</i> Q neuroblast lineage during migration and differentiation.

ABSTRACT: Proper migration and differentiation of neuroblasts into neurons are essential for the development of a healthy nervous system. In this context, the asymmetrical migration of Caenorhabditis elegans Q neuroblasts provides a powerful model for studying the genetic aspects of neuronal migration in vivo at single-cell resolution. We isolated Q lineage cells at various stages of development using FACS and employed single-cell RNA sequencing to investigate the molecular mechanisms underlying the migration and differentiation of these neuroblasts. We created a robust transcriptomic differentiation map of the Q neuroblast lineage and used established markers to identify each cell in the lineage. Our results revealed novel genes not previously described on these cells and linked the e

SUBMITTER: Teixeira FL 

PROVIDER: S-EPMC12439895 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature