Engineered histones reshape chromatin in human cells.

ABSTRACT: Histone proteins and their variants have been found to play crucial and specialized roles in chromatin organization and the regulation of downstream gene expression; however, the relationship between histone sequence and its effect on chromatin organization remains poorly understood, limiting our functional understanding of sequence variation between distinct subtypes and across evolution and frustrating efforts to rationally design synthetic histones that can be used to engineer specified cell states. Here, we make the first advance towards engineered histone-driven chromatin organization. By expressing libraries of sequence variants of core histones in human cells, we identify variants that dominantly modulate chromatin structure. We further interrogate variants using a combination of im

SUBMITTER: Jena SG 

PROVIDER: S-EPMC12439981 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature