Assembly of the mitochondrial outer membrane module of the trypanosomal tripartite attachment complex.

ABSTRACT: The parasitic protozoan Trypanosoma brucei has a single mitochondrial nucleoid, anchored to the basal body of the flagellum via the tripartite attachment complex (TAC). The detergent-insoluble TAC is essential for mitochondrial genome segregation during cytokinesis. The TAC assembles de novo in a directed way from the probasal body towards the kDNA. However, the OM TAC module which is composed of five subunits, has previously been suspected to follow more complicated assembly pathways. Here, we identified four detergent-soluble OM TAC module subcomplexes that we assign to two classes. One class contains an oligomeric TAC40 complex that according to AlphaFold contains 6-8 subunits, as well as two subcomplexes of different sizes comprising TAC40, TAC42, and TAC60. The second class consists o

SUBMITTER: Stettler P 

PROVIDER: S-EPMC12440218 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature