MiR‑27b‑3p modulates CD4+CD39+ Tregs to drive immune‑mediated intervertebraldisc degeneration.

ABSTRACT: Intervertebral disc degeneration (IVDD) is a major cause of chronic back pain, yet its molecular mechanisms remain poorly understood despite its high prevalence. In the present study, the significant role of microRNA (miR)‑27b‑3p in regulating 731 immune cell types was systematically uncovered utilizing Mendelian randomization (MR) and single‑cell RNA sequencing, with a particular focus on CD4⁺CD39⁺ regulatory T cells (Tregs); and its critical impact on immune‑mediated IVDD progression was highlighted. A total of 76 miRs were screened and, through MR analysis, a significant causal relationship between miR‑27b‑3p and IVDD was identified. Subsequent in vivo and in vitro experiments demonstrated that miR‑27b‑3p overexpression not only promoted apoptosis of

SUBMITTER: Li Q 

PROVIDER: S-EPMC12440270 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature