Cyclic-di-GMP signalling mutants drive ecological succession and self-generated diversity in experimentally evolved biofilms of <i>Pseudomonas aeruginosa</i>.

ABSTRACT: Biofilms represent a discrete form of microbial life which are physiologically distinct from free-living planktonic cells. The altered phenotypic manifestations of the biofilm may also elicit lifestyle-dependent adaptive responses to selective pressures. In this work, an experimental evolution model was used to study the adaptation to a biofilm lifestyle in Pseudomonas aeruginosa PA14. The serial passage of biofilms selected for biofilm hyperproduction in a stepwise fashion characterized by increased biomass production and phenotypic diversification was not associated with reduced susceptibility to antibiotics. Adaptation to a biofilm lifestyle selected for mutations causes constitutive increases of intracellular c-di-GMP concentrations via mutations in the phosphodiesterase dipA

SUBMITTER: Wickham GJ 

PROVIDER: S-EPMC12440571 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature