Mechanism of IFITM1 regulating epidural scar hyperplasia after laminectomy through SMAD3/CBR4 pathway.

ABSTRACT:

Background

Epidural scar hyperplasia is a prevalent complication post-laminectomy, contributing significantly to persistent low back pain and other symptoms, ultimately undermining surgical outcomes. Previous studies have identified fibroblast proliferation and differentiation, as well as adipocyte fibrosis, as central to this process, though the precise mechanisms remain elusive.

Methods

A model of laminectomy was established using wild-type mice and IFITM1-KO mice. Methods such as HE staining and Masson staining were employed to assess the degree of fibrosis in the postoperative wound area of the mice. Immunofluorescence and Western blot were performed to verify the localization of IFITM1 and fibronectin. NIH-3T3 fibroblast cells and primary fibroblast cell models were est

SUBMITTER: Wang H 

PROVIDER: S-EPMC12440877 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature