Multi-omics integration uncovers key transcriptional regulators in triple-negative breast cancer spatial heterogeneity.

ABSTRACT:

Background

Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer characterized by a lack of hormone receptors, making it challenging to treat.

Methods

We generated a comprehensive spatial cell atlas of TNBC using a multi-omics integration approach that combined single-cell RNA sequencing (scRNA-seq) with spatial transcriptomics. This integration allowed us to characterize the spatial microenvironment and map the cell-type-specific distributions in TNBC tissues.

Results

Our analysis revealed significant heterogeneity in cell types and spatial distribution, with normal regions enriched in insulin resistance functions, whereas cancerous regions displayed diverse cell populations, including immune cells, cancer-associated fibroblasts (CAFs), and m

SUBMITTER: Zhang N 

PROVIDER: S-EPMC12440963 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature