CENP-A is diluted during bovine spermatogenesis and is maintained at internally positioned centromere clusters in mature bull sperm.

ABSTRACT: During spermatogenesis, chromatin structure is remodelled by the incorporation of distinct histone variants and associated posttranslational modifications, followed by the almost complete replacement of histones by protamines in sperm. However, the dynamics of the centromere-specific histone H3 variant CENP-A have not yet been elucidated during spermatogenesis in mammals. Here we investigate CENP-A localisation dynamics in cattle (Bos taurus). In bovine testis tissue sections, we quantify CENP-A intensity in key germ cell types; spermatogonia (pre-meiotic), primary spermatocytes (meiotic) and spermatids (post-meiotic). Our quantitation shows that spermatogonia harbour the highest amount of CENP-A compared to all other germ cell types. Spermatids have approximately one quarter the amount of

SUBMITTER: Stiavnicka M 

PROVIDER: S-EPMC12441075 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature