A novel scorpine-like peptide from the amazonian scorpion <i>Brotheas amazonicus</i> with cytolytic activity.

ABSTRACT:

Introduction

Scorpion venoms contain bioactive molecules with potential antitumor properties. This study aimed to evaluate the cytotoxic effects of crude Brotheas amazonicus venom (BamazV) and its molecular weight-separated fractions on human breast cancer cell lines, with a focus on identifying active compounds and elucidating their mechanisms of action.

Methods

Human breast epithelial (MCF10A) and breast cancer cell lines (SKBR3, MCF7, and MDA-MB-231) were first assessed for dose-dependent responses to paclitaxel, a standard chemotherapeutic agent. BamazV was fractionated by ultrafiltration into >10 kDa, 3-10 kDa, and <3 kDa fractions, which were tested for cytotoxic activity. The active fraction underwent reversed-phase chromatography, and the major bioactive pepti

SUBMITTER: Reis MB 

PROVIDER: S-EPMC12441280 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature