Mesenchymal stromal cell secretome-induced synaptogenesis is mediated by thrombospondin-1.

ABSTRACT: Mesenchymal stromal cells (MSCs) have been proposed as a promising therapeutic tool for traumatic disorders of the nervous system, due to their neuro-regenerative properties. These outcomes have been extensively connected to paracrine mechanisms. Because synaptic reintegration of injured axons into existing circuitry is required for functional recovery, we investigated the synaptogenic potential of MSC secretome. Using neuronal primary cultures, hippocampal organotypic slices, and human cortical brain organoids, we found that MSC secretome induces presynaptic differentiation. Moreover, the number of axodendritic synapses also increases after treatment with MSC secretome. This increase in synapses correlates with an enhancement in synaptic activity, revealing that newly formed synapses are

SUBMITTER: Tome D 

PROVIDER: S-EPMC12441700 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature