The role of focal adhesion kinase in bladder cancer: translation from <i>in vitro</i> to <i>ex vivo</i> human urothelial carcinomas.

ABSTRACT:

Background

Focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase, plays a crucial role in focal adhesion turnover by interfacing between the extracellular space, transmembrane integrins, and actin filaments. Its significance for the progression of several malignancies, including bladder cancer, has been well-documented. However, its precise role and the implications of its inhibition in bladder cancer tissues and urothelial in vitro models has not been fully explored. This study examined FAK expression and function in human bladder cancer biopsies and in vitro bladder cancer models.

Materials and methods

Ex vivo analyses were performed using reverse transcription-quantitative PCR (qRT-PCR), western blotting, and immunohistochemistry to compare FAK e

SUBMITTER: Markovic G 

PROVIDER: S-EPMC12441887 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature