PagARF3.1 promotes adventitious root formation by repressing <i>IPT</i>-mediated cytokinin biosynthesis.

ABSTRACT: Adventitious root formation is a crucial developmental process in the clonal propagation of economically significant horticultural and woody species. This process is antagonistically regulated by auxin and cytokinin, yet the underlying molecular regulatory mechanisms remain poorly understood. In this study, we investigated the role of PagARF3.1, a homolog of Arabidopsis auxin response factor 3, in hybrid poplar (Populus alba × Populus glandulosa clone cv. '84K'), focusing on its involvement in adventitious root formation. GUS staining analysis revealed that PagARF3.1 was expressed in adventitious root tips, pericycle cells, early root primordia, and outgrowing roots. Knockdown of PagARF3.1 delayed adventitious root formation and reduced root biomas

SUBMITTER: Liu YL 

PROVIDER: S-EPMC12442033 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature