Quantification of Small Molecule Partitioning in a Biomolecular Condensate with 2D Nuclear Magnetic Resonance Spectroscopy.

ABSTRACT: Several intrinsically disordered proteins have been shown to undergo phase separation into a dense and dilute phase and this process is intimately linked with the regulation of cellular processes. It is therefore highly relevant to know how metabolites partition between these phases. It is shown here that the partitioning of components in a complex mixture can be robustly obtained from a single set of 2D nuclear magnetic resonance (NMR) spectra recorded on the dilute and dense phases separately using "time-zero extrapolated" heteronuclear single quantum coherence (HSQC₀) spectroscopy. The spectral separation power of 2D NMR spectroscopy circumvents the need for physical isolation or workup of the mixture components in the two samples. Using quantitative 1D ¹H NMR, it

SUBMITTER: Buhl JM 

PROVIDER: S-EPMC12442244 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature