CTPS2 regulates CTP synthetase activity by interacting with CTPS1.

ABSTRACT: CTP synthetases (CTPS) 1 and 2 are responsible for intracellular de novo production of the CTP nucleotide required for DNA replication and cell proliferation. CTPS1 and CTPS2 are co-expressed in most of tissues and share a high structural homology. They form homotetramers that are enzymatically active and aggregate into large intracellular storage filament-like structures termed as cytoophidia. Herein, we found that CTPS1 and CTPS2 co-localized in cytoophidia when co-expressed and CTPS2-containing filaments are dependent on CTPS1 expression. Cytoophidia were not necessary for proliferation because CTPS1^H355A and CTPS2^H355A mutants that are unable to form cytoophidia could sustain normal cell proliferation. CTPS1 and CTPS2 were found to directly interact together indep

SUBMITTER: Minet N 

PROVIDER: S-EPMC12442307 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature