Tracing of streptococcal strains from infant stools across human body sites links site-specific prevalence to adhesins.

ABSTRACT: Streptococci colonize various human body sites, both as commensals and as pathogens. They are early gut colonizers, but we lack strain-level information about the origin and persistence of streptococci in the non-diseased gut. To gain a greater insight into commensal streptococci in the infant gut and their presence in other body habitats, we analyzed samples from mother-infant pairs collected from multiple body sites (stool, oral cavity, vagina, breast milk). We performed whole-metagenome sequencing and isolated streptococci from 100 infant stool samples (collected at 10 days of age). To trace the streptococci at the strain level, we designed selective quantitative PCR (qPCR) primers for seven streptococcal strains, which were then used to screen the corresponding samples from the other b

SUBMITTER: Ormaasen I 

PROVIDER: S-EPMC12442395 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature