Genome evolution of <i>Acinetobacter baylyi</i> ADP1 during laboratory domestication: acquired mutations impact competence and metabolism.

ABSTRACT: The bacterium Acinetobacter baylyi is a model organism known for its extreme natural competence and metabolic versatility. It is capable of taking up environmental DNA at a high rate across all growth phases. The type strain ADP1 was created by random mutagenesis of a precursor strain, BD4, to prevent it from forming cell chains in culture. ADP1 has since been distributed between research groups over several decades and acquired subsequent mutations during this time. In this study, we compare the genome sequences of A. baylyi BD4 and its modern descendants to identify and understand the effects of mutations acquired and engineered during its domestication. We demonstrate that the ADP1 variants in use today differ in their competence, growth on different carbon sources, and au

SUBMITTER: Gifford I 

PROVIDER: S-EPMC12442408 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature