Detection and Characterization of Cyanometabolite Complexes between Microcystin-RR and a Microguanidine by UHPLC-ESI-MS.

ABSTRACT: Microcystins (MCs) are hepatotoxic cyclic peptides produced by cyanobacteria, with MC-RR being one of the most polar and commonly detected MC congeners in water collected during cyanobacterial harmful algal blooms (cHABs). Microguanidines (MGDs) are sulfated metabolites produced by Microcystis sp. that have not been reported during Lake Erie cHABs. Herein, both MGD AL772 (also abbreviated here as MGD) and previously unreported complex ions containing MC-RR and MGD AL772 were discovered after UHPLC-ESI-HRMS, MS/MS, and MS/MS/MS analyses of the Lake Erie cHAB samples in positive and negative modes. Initially, a triply charged [(MC-RR)₂-MGD+3H]³⁺ ion was detected at the same retention time as coeluting [MC-RR+2H]²⁺ and [MGD-H]^- ions. Characte

SUBMITTER: Premathilaka SH 

PROVIDER: S-EPMC12442505 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature