Construction of an engineered <i>Bacillus subtilis</i> for production of poly-γ-glutamic acids with specific molecular weights.

ABSTRACT:

Introduction

Poly-γ-glutamic acid (γ-PGA) with different molecular weight (Mw) exhibits different properties and therefore has a variety of applications. At present, the γ-PGA is mainly produced by Bacillus species. However, the production of γ-PGAs with specific Mws often requires multiple strains, which limits the development and application of γ-PGA.

Methods

To address this limitation, we constructed an engineered Bacillus subtilis strain by deleting hydrolase genes cwlO, pgdS and ggt, and further introduced regulation of PgdS expression under an IPTG-inducible promoter.

Results

When the hydrolase genes cwlO, pgdS and ggt in B. subtilis were deleted, the γ-PGA Mw and titer increased by 220.1% (2.42×10

SUBMITTER: Lin Y 

PROVIDER: S-EPMC12442558 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature