Mouse model validity for studying the impact of tobacco smoke on the human gut microbiota assessed via in silico and experimental approaches.

ABSTRACT:

Introduction

The contribution of cigarettes to disease initiation, manifestation and progression is well-established for complex disorders, such as inflammatory bowel disease. However, studying its impact on disease pathophysiology in a controlled setting is challenging in humans, resulting in the application of various model systems, amongst them tobacco smoke-exposed mice. While frequently employed, it is unclear to what extent this model reflects human responses to tobacco smoke.

Methods

Employing a mouse study of experimental nature, we assessed established parameters for monitoring responses to tobacco smoke, paralleled by 16S rRNA gene-based profiling of the murine gut microbiome in n=32 suitable animals. This was supplemented by a case-control study design, based on n

SUBMITTER: Bermudez-Perez IV 

PROVIDER: S-EPMC12442884 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature