A comprehensive analysis of allele-specific expression and transcriptomic profiling in pig limbic and endocrine tissues.

ABSTRACT:

Introduction

Stress involves complex interactions between the brain and endocrine systems, but the gene-level processes and genetic factors mediating these responses remain unclear. This study investigates gene expression patterns and allele-specific expression (ASE) in key limbic, diencephalon and endocrine tissues to better understand stress adaptation at the molecular level.

Methods

We performed RNA sequencing on 48 samples from six distinct tissues: amygdala, hippocampus, thalamus, hypothalamus, pituitary gland, and adrenal gland. These tissues were categorized into three functionally and anatomically distinct groups: limbic (amygdala, hippocampus), diencephalon (thalamus, hypothalamus), and endocrine (pituitary, adrenal). Differential expression analyses were conducted

SUBMITTER: Iqbal MA 

PROVIDER: S-EPMC12442888 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature