Genomic characterization and fermentation study of the endophyte Stemphylium sp. (Aa22), a producer of bioactive alkyl-resorcinols.

ABSTRACT: The genome of the previously described endophytic fungus Stemphylium sp. (strain Aa22) has been sequenced to near completion. Phylogenomic analysis placed strain Aa22 in close proximity to Stemphylium lycopersici. Strain Aa22 had been previously reported as the producer of the bioactive alkyl-resorcinol stemphol and derivative stempholones A and B in solid culture on rice. Genome mining for biosynthetic gene clusters (BGCs) identified 38 genomic regions predicted to encode secondary metabolites production. Among them, a single type III polyketide synthase (T3PKS) that shared similarity with other fungal T3PKSs was identified. T3PKSs are responsible for the biosynthesis of alkyl-resorcinols from fatty acyl-CoA substrates. This makes the identified T3PKS gene a likely candidate for stempholo

SUBMITTER: Rojas Lopez-Menchero J 

PROVIDER: S-EPMC12443245 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature