Impact of maximal overexpression of a non-toxic protein on yeast cell physiology.

ABSTRACT: While it is recognized that excess expression of non-essential proteins burdens cell growth, the physiological state of cells under such stress is largely unknown. This is because it is challenging to distinguish between adverse effects arising from the properties of the expressed excess protein (cytotoxicity) and those caused solely by protein overexpression. In this study, we attempted to identify the model protein with the lowest cytotoxicity in yeast cells by introducing a new neutrality index. We found that a non-fluorescent fluorescent protein (mox-YG) and an inactive glycolytic enzyme (Gpm1-CCmut) showed the lowest cytotoxicity. These proteins can be expressed at levels exceeding 40% of total protein while maintaining yeast growth. The transcriptome of cells expressing mox-YG to the

SUBMITTER: Fujita Y 

PROVIDER: S-EPMC12443478 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature