Characterization of an induced pluripotent stem cell line (NCHi024-A) from a male infant with bicuspid aortic valve.

ABSTRACT: Bicuspid aortic valve is one of the most common congenital heart defects which have only two leaflets instead of three. NCHi024-A is an induced pluripotent stem cell (iPSC) line derived from peripheral blood mononuclear cells (PBMCs) of a male infant with a bicuspid aortic valve. The line exhibits normal iPSC morphology, normal karyotype, and donor-matched identity. Markers of undifferentiated iPSC and differentiation into ectoderm, mesoderm, and endoderm were validated through immunofluorescence staining. NCHi024-A was tested negative for transgenes and mycoplasma contamination. This iPSC line can be differentiated into various types of cells and used for studying aortic valve disease.

SUBMITTER: Lee J 

PROVIDER: S-EPMC12443494 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature