Circulating tumor DNA as a marker of molecular residual disease in resected esophageal squamous cell carcinoma.

ABSTRACT: Esophageal squamous cell carcinoma (ESCC) remains a major contributor to cancer-related mortality, with molecular residual disease (MRD) detection posing a significant challenge in post-surgical management. This study aimed to evaluate the effectiveness of circulating tumor DNA (ctDNA) in detecting MRD in patients with resectable ESCC undergoing radical surgery. A total of 62 primary tumor tissues, 108 preoperative, and 125 postoperative plasma samples were collected from 125 such ESCC patients who underwent radical surgery, and subjected to sequencing. Next-Generation Sequencing and ultra-high sensitivity Automated Triple Groom Sequencing panels were used to sequence genomic DNA from tumor tissues and ctDNA from plasma, respectively. ctDNA positive mutations included tumor-informed mutati

SUBMITTER: Fang CY 

PROVIDER: S-EPMC12443647 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature