Multifunctional cerium nanolabels in electrochemical immunosensing with improved robustness and performance: determination of TIM-1 in colorectal cancer scenarios as a case study.

ABSTRACT: A multifunctional cerium oxide nanoparticles (CeO₂NPs)-based nanolabel is exploited to implement an electrochemical sandwich-type immunoplatform for the determination of T-cell immunoglobulin and mucin domain 1 (TIM-1) biomarker, a mucin-like class I membrane glycoprotein associated with cancer angiogenesis. The immunoplatform is constructed using screen-printed electrodes where capture antibody is immobilized through the chemistry of diazonium salts. CeO₂NPs exhibit robust pseudo-peroxidase activity even at high substrate concentrations. They are covalently functionalized in a simple manner after carboxylation with a detector antibody (dAb), acting dually as a nanozyme and nanocarrier for sensing bioreceptors. This allows the development of immunoplatforms with impro

SUBMITTER: Cabrero-Martin A 

PROVIDER: S-EPMC12443898 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature