Unresolved ER stress restricts in vitro plant cell totipotency.

ABSTRACT:

Key message

Many plant cells can be induced to regenerate in vitro. We show that successful regeneration during microspore-derived embryo culture relies in part on the ability of embryogenic cells to resolve tissue culture-induced ER stress. During Brassica napus microspore embryogenesis, the immature male gametophyte is induced by a heat stress treatment to develop into a haploid embryo. Different multicellular embryogenic structures develop in response to heat stress, each with a different potential to complete embryo development. The underlying factors that determine the ability of these initially embryogenic structures to successfully complete embryo development are not known. We show that all embryogenic structures exhibit elements of endoplasmic reticulum (ER) stress, like ER

SUBMITTER: Martinez PC 

PROVIDER: S-EPMC12443906 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature