Tri-domain proteins 27 alleviates ischemia-reperfusion injury-induced acute kidney injury by promoting Gli-like transcription factor 1 expression via the inhibition of polycomb repressive complex 2 activity.

ABSTRACT: Dedifferentiated renal tubular epithelial cell (RTEC) proliferation contributes to renal repair following acute kidney injury (AKI) induced by renal ischemia-reperfusion (I/R) injury (RIRI). However, the fundamental mechanism underlying RTEC dedifferentiation remains unclear. An animal model of RIRI-induced AKI was established using I/R, and H₂O₂-treated murine (m) RTECs were used as the cell injury model. Pathological changes were assessed by hematoxylin and eosin and periodic acid-Schiff stain staining. Cell viability and migration were assessed using the cell counting kit-8 and wound healing assays, respectively. Apoptosis was examined using flow cytometry. Molecular interactions were investigated using coimmunoprecipitation and chromatin IP assays. Tri-domain prot

SUBMITTER: Xiong C 

PROVIDER: S-EPMC12444411 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature