Intermittent Fasting Reduces Obesity-driven Oxidative Stress in the Male Mouse Colon via Changes in Gut Microbiota.

ABSTRACT:

Background & aims

Obesity impairs colon homeostasis by increasing oxidative stress, which is associated with damage to the epithelial barrier and the development of intestinal diseases. Intermittent fasting (IF) improves mitochondrial activity (MA) in metabolic tissues and positively modifies gut microbiota composition, suggesting that this intervention may exert beneficial effects on colon homeostasis. The aim of this study was to evaluate the effect of IF on MA in the colon and its modulation by gut microbiota in a diet-induced obesity mouse model.

Methods

C57BL/6 male mice were fed a control diet or high-fat/high-sucrose diet (HFSD) for 12 weeks, followed by 4 weeks of IF intervention or continued HFSD, with or without antibiotic (AB) treatment. We evaluated MA in colonic

SUBMITTER: Condado-Huerta C 

PROVIDER: S-EPMC12444470 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature