Continuous β^- particle exposure: A study of DNA damage in <i>ex vivo</i> peripheral blood mononuclear cells irradiation with Radioiodine.

ABSTRACT:

Background and purpose

Targeted radionuclide therapy (TRT) is a promising cancer treatment, but insufficient knowledge of the biological dose-response relationships limits its efficacy. The aim of this study was to characterize DNA damage in peripheral blood mononuclear cells (PBMCs) exposed to β-continuous irradiation (¹³¹I), and to assess its relationship with the absorbed dose, using two biomarkers: dicentric chromosomes and γ-H2AX foci.

Materials and methods

PBMCs from healthy donors were exposed to ¹³¹I at various activities (0.37-3.7 MBq) and times (1, 4, 24 h). Absorbed doses were calculated using the Medical Internal Radiation Dose formalism. DNA damage was assessed by chromosomal aberration frequency and γ-H2AX foci quantification. Lithium chlo

SUBMITTER: Mazzitelli-Fuentes L 

PROVIDER: S-EPMC12444485 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature