Analytical Development for Detecting Femtomole-Level Organophosphorus Compounds in Biogeochemical Samples by Ion Chromatography/High-Resolution Mass Spectrometry.

ABSTRACT: Phosphorus (P) is a crucial element for life, and its organic forms play a central role in modern biology. Therefore, the analysis of their presence in environmental samples is important for evaluating the biogeochemistry of P and the P cycle on the Earth. Herein, we developed a high-sensitivity, high-resolution analytical technique for detecting organophosphorus compounds using ion chromatography (IC) with Orbitrap-mass spectrometry (Orbitrap-MS). The IC method clearly separated organophosphorus compounds, including alkyl phosphonates and alkyl phosphates, from other organic molecules and inorganic ions. The online combination of IC and Orbitrap-MS enabled the quantification of organophosphorus compounds with concentrations as low as ∼10 fmol and their molecular identification in the pres

SUBMITTER: Hirakawa Y 

PROVIDER: S-EPMC12444523 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature