Physicochemical Properties of Phosphoramide <i>N</i>‑Benzimidazole Oligonucleotides in the Presence of Cosolvents.

ABSTRACT: Phosphoramide benzoazole oligonucleotides (PABAOs) are a recently developed class of phosphate-modified nucleic acid derivatives. In this study, we investigated the physicochemical properties of N-benzimidazole oligodeoxyribonucleotides and their duplexes with complementary DNA in the presence of low-molecular-weight cosolvents. For this purpose, we analyzed the effects of ethanol (EtOH), ethylene glycol (EG), glycerol, trifluoroethanol (TFE), and dimethyl sulfoxide (DMSO) on the structure, dynamics, hybridization properties, and solvation of PABAOs. The presence of 20% cosolvents did not alter the secondary structures of native or modified oligonucleotides and duplexes, as confirmed by circular dichroism data. Molecular dynamics simulations revealed structural similarities between

SUBMITTER: Morozova FV 

PROVIDER: S-EPMC12444524 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature