Loading Proteins into Extracellular Vesicles to Camouflage Protein Allergens.

ABSTRACT: Extracellular vesicles (EVs) are cell-derived membranous nanoparticles with a high potential as drug delivery systems due to their intrinsic capability to vehicle biological materials and information. Beyond the transport of small drugs and therapeutics, a biotechnological challenge consists of loading nanoparticles and macromolecules with large size, including antibodies and other proteins. Here, we use microalgal-derived EVs, named nanoalgosomes, that are biocompatible, sustainable, and green EVs derived from microalgae and thoroughly characterized in our previous work, and a recombinant calcium-binding protein (CBP), Par j 4, a minor allergen from Parietaria judaica (Pj). We set up an efficient method to load Par j 4 into nanoalgosomes, using extrusion as the loading techn

SUBMITTER: Rao E 

PROVIDER: S-EPMC12444598 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature